We developed software for single-nucleotide polymorphism detection in Aspergillus nidulans, described in Tan et al. 2014. Please see this file for a description of the pipeline. We have written four pieces of code that are required for the pipeline. They can be downloaded below.
These protocols describe in detail the methods used in: Derr ND*, Goodman BS*, Jungmann R, Leschziner AE, Shih WM, Reck-Peterson SL. (2012) Tug of war in motor protein ensembles revealed with a programmable DNA origami scaffold. Science 338: 662-665.
- DNA origami scaffold preparation
- 12 helix bundle DNA origami folding and purification
- Attaching the SNAP tag ligand to oligonucleotides and purification
- Labeling kinesin with oligonucleotide-linked SNAP tag ligands
Additional information can be found in the supplemental material of our paper [pdf].